Journal: Cell Death Discovery

Article Title: Neuroprotective effect of astragalin via activating PI3K/Akt-mTOR-mediated autophagy on APP/PS1 mice

doi: 10.1038/s41420-023-01324-1

Figure Lengend Snippet: A Cell viability was detected in HT22 cells with different concentrations of Aβ25-35 (0–160 μM Aβ25-35, 24 h) by CCK8 assay. B Cell viability was detected in Aβ25-35-injured HT22 cells (20 μM Aβ25-35, 24 h) with different concentrations of AST (4 h before Aβ25-35 treatment) by CCK8 assay. C Cell viability was detected in HT22 cells with different concentrations of AST (0–160 μM AST, 24 h) by CCK8 assay. Data were presented as mean ± SD, * p < 0.05 vs 0 μM . D Effect of AST on the morphological alteration of HT22 cells was observed by immunofluorescent staining. E , F Flow cytometry analysis indicated the anti-apoptotic effect of AST on the apoptosis of HT22 cells. Data were presented as mean ± SD, n = 3/group. *** p < 0.001 vs control group, ## p < 0.01 vs AD group, @ p < 0.05 vs AST + AD group.

Article Snippet: And then the data were analyzed by flow cytometry software (Becton-Dickinson, CA, USA).

Techniques: CCK-8 Assay, Staining, Flow Cytometry, Control

Journal: International Journal of Molecular Sciences

Article Title: mtDNA as a Mediator for Expression of Hypoxia-Inducible Factor 1α and ROS in Hypoxic Neuroblastoma Cells

doi: 10.3390/ijms18061220

Figure Lengend Snippet: Hypoxia and mitochondrial DNA (mtDNA) affect the production of ROS and loss of mitochondrial membrane potential (Δψ m ). Intracellular H 2 O 2 production ( A ); or mitochondrial superoxide ( B ) under normoxia or after hypoxia for 4 h were determined by flow cytometry with DCFH-DA or MitoSox red individually in both SK-N-AS and ρ 0 cells; ( C ) Loss of mitochondrial membrane potential in normoxic or hypoxic SK-N-AS and ρ 0 cells were measured by Rodamine 123. The percentages loss of Δψm indicated the number of Δψm collapsed cells after exposure to hypoxia. These results are shown as mean ± SD of more than three independent experiments. Statistical significance (* p < 0.05, # p < 0.005) was determined using the one-way ANOVA compared to normoxia.

Article Snippet: The data was analyzed later with using the flow cytometry analysis software BD CellQuest (Becton Dickinson, San Jose, CA, USA), by gated 10 2 –10 4 area.

Techniques: Membrane, Flow Cytometry